Part:BBa_K4286099:Design
Classical oscillator composed of three genes encoding repressor protein
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2188
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
How to ensure the safety of engineered microorganisms for agricultural applications is a serious issue. In order to prevent the engineered microorganisms from escaping, it is a feasible method to use the conditional triggered suicide switch. However, this is not foolproof, because the triggering conditions in the natural environment are uncertain, and there may still be the possibility of escaping. 2022 SZU-China attaches great importance to the safety of engineered microorganisms and hopes to design a suicide switch activated by endogenous factors.
Based on the classical gene oscillator, we propose a concept of timed suicide switch, hoping to kill the engineered microorganism population at a certain time. Based on the classical model organism Escherichia coli, we have designed and improved the gene circuit. We also explore the properties of the timed suicide switch through a mathematical model.
Source
This is a classical oscillator, see Ref.
References
[1]Elowitz MB, Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature. 2000 Jan 20;403(6767):335-8. doi: 10.1038/35002125. PMID: 10659856.
[2]Purcell O, di Bernardo M, Grierson CS, Savery NJ. A multi-functional synthetic gene network: a frequency multiplier, oscillator and switch. PLoS One. 2011 Feb 17;6(2):e16140. doi: 10.1371/journal.pone.0016140. PMID: 21359152; PMCID: PMC3040778.
[3]Potvin-Trottier L, Lord ND, Vinnicombe G, Paulsson J. Synchronous long-term oscillations in a synthetic gene circuit. Nature. 2016 Oct 27;538(7626):514-517. doi: 10.1038/nature19841. Epub 2016 Oct 12. PMID: 27732583; PMCID: PMC5637407.